Stem Cell

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    Protocols in Current Issue
    Primary Culture System for Germ Cells from Caenorhabditis elegans Tumorous Germline Mutants
    Authors:  Alexandra S. Vagasi, Mohammad M. Rahman, Snehal N. Chaudhari and Edward T. Kipreos, date: 08/05/2017, view: 4867, Q&A: 0
    [Abstract] The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous mutants can be fully cellularized and isolated intact from the mutant animals. ...
    Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish
    Authors:  Toshihiro Kawasaki and Noriyoshi Sakai, date: 11/05/2016, view: 4565, Q&A: 0
    [Abstract] Allogeneic organ transplantation is a powerful tool for clinical and basic research studies. However, the graft is often rejected by the host organism. Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish. These zebrafish escaped rejection, which made it possible to successfully transplant fragments of an ...



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