Cancer Biology

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    Protocols in Current Issue
    Immunofluorescent Staining of Claudin-2 in Cultured Kidney Tubular Cells
    Authors:  Shaista Anwer and Katalin Szaszi, date: 07/20/2020, view: 1483, Q&A: 0
    [Abstract] Members of the claudin family of tight junction proteins regulate paracellular permeability and modulate cell signaling. During junction remodeling, these proteins are selectively inserted into or retrieved from the tight junctions, but the control and coordination of these processes remain incompletely understood. Visualization of claudins allows ...
    Isolation of Microvascular Endothelial Cells
    Authors:  Kenneth C.P. Cheung and Federica M. Marelli-Berg, date: 06/20/2018, view: 11470, Q&A: 0
    [Abstract] The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. Murine endothelial cell culture is an important tool for cardiovascular disease research. This protocol demonstrates a quick, efficient method for the isolation of microvascular endothelial cells from murine ...
    Mouse Model of Dextran Sodium Sulfate (DSS)-induced Colitis
    Authors:  Srustidhar Das, Surinder K. Batra and Satyanarayana Rachagani, date: 08/20/2017, view: 13969, Q&A: 0
    [Abstract] Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the intestinal tract and is primarily comprised of Crohn’s disease (CD) and ulcerative colitis (UC). Several murine models that include both chemical induced and genetic models have been developed that mimic some aspects of either CD or UC. These models have been instrumental in ...
    Induction of Colitis and Colitis-associated Colorectal Cancer (CAC)
    Authors:  Jalaj Gupta and Angel R. Nebreda, date: 11/20/2014, view: 13095, Q&A: 0
    [Abstract] Inflammatory bowel disease (IBD) including Crohn’s disease and ulcerative colitis are characterized by chronic, progressive and relapsing inflammatory disorders. Existing evidence indicate that IBD is associated with a higher risk of developing CAC, which is directly related to the duration and extent of colitis. Thus, animal models have been ...
    Vaccine-induced Cytokine Production Detected by Luminex Multiplex Analysis
    [Abstract] Different vaccine and adjuvant combinations are known to rapidly induce antigen presenting cell (APC) maturation and pro-inflammatory cytokine and production, which in turn play an important role in the priming of antigen-specific T cells. Measuring cytokine production systemically in the serum fails to detect localized responses in the lymph ...
    Xenograft Tumor Growth Assay
    Authors:  Li-Ting Wang and Shih-Hsien Hsu, date: 10/20/2013, view: 11969, Q&A: 0
    [Abstract] Chronic inflammation drives initiation of hepatocellular carcinoma (HCC), but the underlying mechanisms linking inflammation and tumor formation remain obscure. In this study, Xenograft tumor assay was used to determine the tumorigenic activity of hepatoma cells with ISX over expression on nude mice in vivo.
    Measurement of Cytokines
    Authors:  Renaud Grépin and Gilles Pagès, date: 11/20/2012, view: 13509, Q&A: 3
    [Abstract] This protocol allows to measure the levels cytokines - such as VEGFs, CXCLs cytokines, PDGF or FGF - from fresh samples but also frozen tumors. The advantage of this method is to use very few micrograms of biological material and the protocol is carried out quickly.



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