Biological Sciences

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    Protocols in Current Issue
    Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts

    The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and

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    Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts
    [Abstract]

    The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and automation were limited by the need to replace pipettes manually after each experiment. This

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    Micrografting in Arabidopsis Using a Silicone Chip
    Authors:  Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi, date: 06/20/2021, view: 1229, Q&A: 0
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

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    Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
    Authors:  Steven Henikoff, Jorja G. Henikoff and Kami Ahmad, date: 06/05/2021, view: 1235, Q&A: 0
    [Abstract]

    We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and single cells. With CUT&Tag, intact cells or nuclei are permeabilized, followed by successive

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    Objective Quantitation of Focal Sweating Areas Using a Mouse Sweat-assay Model
    Authors:  Choongjin Ban and Dae-Hyuk Kwoen, date: 06/05/2021, view: 1012, Q&A: 0
    [Abstract]

    In vivo sweat quantitation assays are required for the development of drugs for the management of focal hyperhidrosis before clinical trials; however, in vivo assays, particularly mouse models, are rare. Even in sweat assays using mice, sweating is quantitated by manually counting the number of sweating spots, which can contribute to various

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    Parasitemia Evaluation in Mice Infected with Schistosoma mansoni
    Authors:  Naiara Clemente Tavares and Marina Moraes Mourão, date: 05/20/2021, view: 1950, Q&A: 0
    [Abstract]

    Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma

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    Operant Vapor Self-administration in Mice
    [Abstract]

    Models of drug addiction in rodents are instrumental in understanding the underlying neurobiology. Intravenous self-administration of drugs in mice is currently the most commonly used model; however, several challenges exist due to complications related to catheter patency. To take full advantage of the genetic tools available to study opioid

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    ODELAM: Rapid Sequence-independent Detection of Drug Resistance in Mycobacterium tuberculosis Isolates
    Authors:  Thurston Herricks, Magdalena Donczew, David R. Sherman and John D. Aitchison, date: 05/20/2021, view: 1624, Q&A: 0
    [Abstract]

    Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths globally each year. Current assays of antimicrobial resistance require knowledge of the mutations that confer drug resistance or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of

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    High-throughput Method for Detecting Siderophore Production by Rhizosphere Bacteria
    Authors:  ShaoHua Gu, Wen Wan, ZhengYing Shao and Wei Zhong, date: 05/05/2021, view: 1623, Q&A: 0
    [Abstract]

    Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated from the rhizosphere, and we used the method presented here to assay the siderophore production by

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    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR
    [Abstract]

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume

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    Age, Wound Size and Position of Injury – Dependent Vascular Regeneration Assay in Growing Leaves
    [Abstract]

    Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we established a new vascular regeneration assay in

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