Plant Science

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    Protocols in Current Issue
    Non-radioactive Assay to Determine Product Profile of Short-chain Isoprenyl Diphosphate Synthases
    Authors:  Avanish Rai and Dinesh A. Nagegowda, date: 01/05/2021, view: 318, Q&A: 0
    Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from ...
    Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa
    Authors:  Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet and Igor Kovalchuk, date: 01/05/2021, view: 414, Q&A: 0

    Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa

    ...
    Stable Transformation of Arabidopsis thaliana Cell Suspension Cultures: A Case Study for the Overexpression of The COI1 Receptor
    Cell suspension cultures have been studied for decades to produce natural molecules. However, the difficulty in generating stably transformed cell lines has limited their use to produce high value chemicals reproducibly and in elevated quantities. ...
    A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia
    Authors:  Xiaofeng Yin and Hirokazu Tsukaya, date: 01/05/2021, view: 249, Q&A: 0
    In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are ...
    Efficient Transient Gene Knock-down in Tobacco Plants Using Carbon Nanocarriers
    Authors:  Gozde S. Demirer and Markita P. Landry, date: 01/05/2021, view: 340, Q&A: 0
    Gene knock-down in plants is a useful approach to study genotype-phenotype relationships, render disease resistance to crops, and enable efficient biosynthesis of molecules in plants. Small interfering RNA (siRNA)-mediated gene silencing is one of ...
    Efficient Transient Gene Knock-down in Tobacco Plants Using Carbon Nanocarriers
    Authors:  Gozde S. Demirer and Markita P. Landry, date: 01/05/2021, view: 340, Q&A: 0
    [Abstract] Gene knock-down in plants is a useful approach to study genotype-phenotype relationships, render disease resistance to crops, and enable efficient biosynthesis of molecules in plants. Small interfering RNA (siRNA)-mediated gene silencing is one of the most common ways to achieve gene knock-down in plants. Traditionally, siRNA is delivered into ...
    A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia
    Authors:  Xiaofeng Yin and Hirokazu Tsukaya, date: 01/05/2021, view: 249, Q&A: 0
    [Abstract] In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are difficult. The few existing methods for detection and quantification of cell division orientation are ...
    Stable Transformation of Arabidopsis thaliana Cell Suspension Cultures: A Case Study for the Overexpression of The COI1 Receptor
    [Abstract] Cell suspension cultures have been studied for decades to produce natural molecules. However, the difficulty in generating stably transformed cell lines has limited their use to produce high value chemicals reproducibly and in elevated quantities.

    In this protocol, a method to stably transform and maintain Arabidopsis cell ...
    Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa
    Authors:  Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet and Igor Kovalchuk, date: 01/05/2021, view: 414, Q&A: 0
    [Abstract]

    Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media. In this protocol, all three

    ...
    Non-radioactive Assay to Determine Product Profile of Short-chain Isoprenyl Diphosphate Synthases
    Authors:  Avanish Rai and Dinesh A. Nagegowda, date: 01/05/2021, view: 318, Q&A: 0
    [Abstract] Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from geranyl diphosphate (GPP; C10), farnesyl diphosphate (FPP; C15), geranylgeranyl ...
    Lipid Droplet Isolation from Arabidopsis thaliana Leaves
    Authors:  Yovanny Izquierdo, Rubén Fernández-Santos, Tomás Cascón and Carmen Castresana, date: 12/20/2020, view: 546, Q&A: 0
    [Abstract]

    Lipid droplets (LDs) are neutral lipid aggregates surrounded by a phospholipid monolayer and specific proteins. In plants, they play a key role as energy source after seed germination, but are also formed in vegetative tissues in response to developmental or environmental conditions, where their functions are poorly understood. To elucidate these,

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    Analysis of Isotopically-labeled Monogalactosyldiacylglycerol Molecular Species from [14C]Acetate-Labeled Tobacco Leaves
    Authors:  Hari Kiran Kotapati and Philip D. Bates, date: 12/20/2020, view: 370, Q&A: 0
    [Abstract]

    Plant lipid metabolism is a dynamic network where synthesis of essential membrane lipids overlaps with synthesis of valuable storage lipids (e.g., vegetable oils). Monogalactosyldiacylglycerol (MGDG) is a key component of the chloroplast membrane system required for photosynthesis and is produced by multiple pathways within the lipid metabolic

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    Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry
    Authors:  Keshun Yu, Huazhen Liu and Pradeep Kachroo, date: 12/05/2020, view: 613, Q&A: 0
    [Abstract]

    Pipecolic acid (Pip), a non-proteinacious product of lysine catabolism, is an important regulator of immunity in plants and humans alike. For instance, Pip accumulation is associated with the genetic disorder Zellweger syndrome, chronic liver diseases, and pyridoxine-dependent epilepsy in humans. In plants, Pip accumulates upon pathogen infection

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    Chromatin Immunoprecipitation (ChIP) to Assess Histone Marks in Auxin-treated Arabidopsis thaliana Inflorescence Tissue
    Authors:  André Kuhn and Lars Østergaard, date: 12/05/2020, view: 704, Q&A: 0
    [Abstract] Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) or high-throughput sequencing (ChIP-seq) has become the gold standard for the identification of binding sites of DNA binding proteins and the localization of histone modification on a locus-specific or genome-wide scale, respectively. ChIP experiments can be divided into seven ...
    Agrobacterium-mediated Transformation of Sweet Basil (Ocimum basilicum)
    Authors:  Natasha Navet and Miaoying Tian, date: 11/20/2020, view: 926, Q&A: 1
    [Abstract]

    Sweet basil (Ocimum basilicum) is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene functional analysis to accelerate molecular breeding and deploy effective disease management strategies, and breeding through genetic engineering. Here we

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