Alessandro Didonna University of California, San Francisco
51 protocols

Ivan Zanoni Harvard Medical School
78 protocols

Jia Li SRI International
51 protocols

Marieta Ruseva GlaxoSmithKline
11 protocols

Reviewer
Sabine Le Saux
  • Research scientist, Merck
Research focus
  • Immunology

Education

PhD, University of Angers, France, 2010

10 Protocols reviewed
A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines
Authors:  Kohei Nishimura and Tatsuo Fukagawa, date: 07/20/2021, view: 830, Q&A: 0

Inducing loss of function of a target protein using methods such as gene knockout is a powerful and useful strategy for analyzing protein function in cells. In recent years, the CRISPR/Cas-9-based gene knockout technology has been widely used across

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Improved Macrophage Isolation from Mouse Skeletal Muscle

Macrophages are a heterogeneous class of innate immune cells that offer a primary line of defense to the body by phagocytizing pathogens, digesting them, and presenting the antigens to T and B cells to initiate adaptive immunity. Through specialized

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