Michael Lynch Duke University Durham
2 protocols

Romel Menacho-Melgar
  • Post-Doc, Department of Biomedical Engineering, Duke University, Durham, NC
Research focus
  • Romel's research focus is using synthetic biology tools to generate advances in protein expression and purification processes
  • 2 Author merit

Education

PhD, Duke University, 2020

Lab information

Michael Lynch lab
https://lynchlab.pratt.duke.edu/

Publications

2 Protocols published
Measuring Oligonucleotide Hydrolysis in Cellular Lysates via Viscosity Measurements
Authors:  Romel Menacho-Melgar and Michael D. Lynch, date: 01/20/2022, view: 1334, Q&A: 0

Cell lysis, a process that releases host oligonucleotides, is required in many biotechnological applications. However, intact oligonucleotides in crude cellular lysates increase the viscosity of lysates, which complicates downstream processes and

...
Simple Scalable Protein Expression and Extraction Using Two-stage Autoinducible Cell Autolysis and DNA/RNA Autohydrolysis in Escherichia coli
Authors:  Romel Menacho-Melgar and Michael D. Lynch, date: 01/20/2022, view: 1344, Q&A: 0

Recombinant protein expression is extensively used in biological research. Despite this, current protein expression and extraction methods are not readily scalable or amenable for high-throughput applications. Optimization of protein expression

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