Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
58 protocols

Karthik Krishnamurthy Thomas Jefferson University
7 protocols

Joshua S Titlow
2 Protocols reviewed
Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot
Authors:  Zaneta Matuszek and Tao Pan, date: 03/20/2019, view: 509, Q&A: 0
Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at ...
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Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture
Authors:  Imre Gaspar, Frank Wippich and Anne Ephrussi, date: 03/05/2018, view: 3379, Q&A: 0
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using ...
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