Imre Gáspár —BIO-PROTOCOL

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Anne Ephrussi Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), Germany
2 protocols

Frank Wippich Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Germany, 69117 Germany,
1 protocol

Abhijit Kale Deciduous Therapeutics
10 protocols

Adam Idoine Carnegie Institution
16 protocols

Alessandro Didonna University of California, San Francisco
48 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

Editor

Imre Gáspár

Faculty, INSTITUTE OF MOLECULAR BIOTECHNOLOGY, IMBA, Vienna

Research focus

Molecular biology
RNA localization, mRNP assembly, cytoskeletal transport,
meiosis, chromatin remodelling during development

2 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Szeged, 2009

Lab information

Kikue Tachibana Lab
https://www.imba.oeaw.ac.at/research/kikue-tachibana/

Publications

https://scholar.google.de/citations?user=I2gbMMwAAAAJ&hl=en

2 Protocols published

Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture

Authors: Imre Gaspar, Frank Wippich and Anne Ephrussi, date: 03/05/2018, view: 8454, Q&A: 0

Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using ...

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Ex vivo Ooplasmic Extract from Developing Drosophila Oocytes for Quantitative TIRF Microscopy Analysis

Authors: Imre Gáspár and Anne Ephrussi, date: 07/05/2017, view: 6291, Q&A: 0

Understanding the dynamic behavior and the continuously changing composition of macromolecular complexes, subcellular structures and organelles is one of areas of active research in both cell and developmental biology, as these changes directly ...

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5 Protocols reviewed

SMART (Single Molecule Analysis of Resection Tracks) Technique for Assessing DNA end-Resection in Response to DNA Damage

Authors: Angela Altieri, Milena Dell’Aquila, Francesca Pentimalli, Antonio Giordano and Alfano Luigi, date: 08/05/2020, view: 2262, Q&A: 0

DNA double strand breaks (DSBs) are among the most toxic lesions affecting genome integrity. DSBs are mainly repaired through non-homologous end joining (NHEJ) and homologous recombination (HR). A crucial step of the HR process is the generation, ...

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Application of Mechanical Forces on Drosophila Embryos by Manipulation of Microinjected Magnetic Particles

Authors: Arturo D’Angelo and Jérôme Solon, date: 05/05/2020, view: 1731, Q&A: 0

Cells generate mechanical forces to shape tissues during morphogenesis. These forces can activate several biochemical pathways and trigger diverse cellular responses by mechano-sensation, such as differentiation, division, migration and apoptosis. ...

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16 Protocols edited

Analyzing (Re)Capping of mRNA Using Transcript Specific 5' End Sequencing

Authors: Daniel del Valle Morales and Daniel R. Schoenberg, date: 10/20/2020, view: 1071, Q&A: 0

The 5′ cap is a ubiquitous feature of eukaryotic mRNAs. It is added in the nucleus onto newly synthesized pre-mRNA, and in the cytoplasm onto mRNAs after decapping or endonuclease cleavage. Cytoplasmic recapping can occur after loss of the cap at ...

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Fluorescence Measurement and Calibration of Intracellular pH in Starfish Oocytes

Authors: Enako Hosoda and Kazuyoshi Chiba, date: 10/05/2020, view: 1324, Q&A: 0

Oocyte maturation is a process wherein an oocyte arrested at prophase of meiosis I resumes meiosis to become a fertilizable egg. In starfish ovaries, a hormone released from follicle cells activates the oocytes, resulting in an increase in their ...

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