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Acute LPS injection

JC Jinbo Cheng
ZY Zengqiang Yuan

Last updated date: Sep 25, 2021 Views: 824 Forks: 0

original An abbreviated version of this protocol was published in Science Advances
Microglial Calhm2 regulates neuroinflammation and contributes to Alzheimer’s disease pathology
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Acute LPS injection:
LPS (0.5 mg/kg; Millipore) administration was done via intraperitoneal injections according to the procedure previously 

described (31). Briefly, mice were treated with a single or double dose of LPS. Each injection was separated by 24 hours. Saline
was injected to the control groups. Whole brains were collected 3 hours after the final injection for immunofluorescence experi-
ments, and hippocampal and cortical tissue were collected for qPCR experiments.

 

In addition, the Supplementary data was attached.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Cheng, J and Yuan, Z(2021). Acute LPS injection. Bio-protocol Preprint. bio-protocol.org/prep1379.
  2. Cheng, J., Dong, Y., Ma, J., Pan, R., Liao, Y., Kong, X., Li, X., Li, S., Chen, P., Wang, L., Yu, Y. and Yuan, Z.(2021). Microglial Calhm2 regulates neuroinflammation and contributes to Alzheimer’s disease pathology. Science Advances 7(35). DOI: 10.1126/sciadv.abe3600

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