Acute LPS injection: LPS (0.5 mg/kg; Millipore) administration was done via intraperitoneal injections according to the procedure previously
described (31). Briefly, mice were treated with a single or double dose of LPS. Each injection was separated by 24 hours. Saline was injected to the control groups. Whole brains were collected 3 hours after the final injection for immunofluorescence experi- ments, and hippocampal and cortical tissue were collected for qPCR experiments.
In addition, the Supplementary data was attached.
Related files
sciadv.abe3600_sm.pdf
Copyright: Content may be subjected to copyright.
How to cite:
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.
0/150
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Spinning
Post a Question
0 Q&A
Spinning
This protocol preprint was submitted via the "Request
a Protocol" track.