Improve Research Reproducibility A Bio-protocol resource
Cell staining
Imaging

How to stain Cell with FITC?,how to visualize leukocyte transmigration by imaging?,how to detect microfilaments of DNA during Netosis process?

ds
dahou sara Aug 5, 2022
webinar Webinar: Advancing single molecule microscopy with active stabilization
  • Neutrophil, NET netosis extracellular traps,MPO myeloproteinase, DNA filaments, HOCl. Neutrophil transmigration a cross endothélium.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

BP-Exchange Assistant Answered Sep 8, 2022

Bio-protocol

Dear Dahou Sara,

thank you for your question. the staining protocol with Fluorescein 5-isothiocyanate (FITC) depends on your approach and organism, there are a number of protocols at bio-protocol.org covering experiments with that stain. Frequently, FITC is used conjugated to an antibody that then recognizes your compound of interest e.g. https://bio-protocol.org/e834

You can also find several protocols covering Netosis, e.g. https://bio-protocol.org/exchange/protocoldetail?id=3927



References

1.
https://bio-protocol.org/e834
2.
https://bio-protocol.org/exchange/protocoldetail?id=3927
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer