How do you ensure reproducibility between MS samples such as prevent disulfide shuffling? What is an appropriate number of replicates to use for MS?

I work with an enzyme that creates disulfide bonds with its substrates. I have developed a protocol to trap my enzyme to its substrate during the process of thiol oxidation using a thiol alkylating agent, N-ethyl-maleimide. This process covalently links the two proteins together via an Read more Read less