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Alkaline Phosphatase
Published: Jul 20, 2012 DOI: 10.21769/BioProtoc.237 Views: 41665
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Abstract
Alkaline phosphatase removes 5' phosphate groups from vector so that prevents self-ligation of the vector and facilitates the ligation of other DNA fragments into the vector.
Background
Materials and Reagents
- Vector DNA
- Restriction enzymes (New England Biolabs)
- Alkaline phosphatase: Calf intestinal alkaline phosphatase (CIP) (New England Biolabs, catalog number: M0290 ) or Shrimp Alkaline Phosphatase (SAP) (Promega Corporation, catalog number: M8201 )
Procedure
Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > DNA cloning
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