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Hi, Could you please explain why we had to heat inactivate the...

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Anaida Walia Oct 20, 2016
protocol Protocol: Alkaline Phosphatase
Hi, Could you please explain why we had to heat inactivate the reaction ?
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Fanglian He Author Answered Oct 22, 2016

Bio-protocol

Hi Anaida,

It is to inactivate alkaline phosphatase otherwise it will continually remove 5' phosphate groups from other DNA fragments, which would affect subsequent ligation step.

--Fanglian
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