Bisulfite sequencing and TAB sequencing

Hi Judit Balog,

Thanks for asking. For the bisulfite sequencing primer for mouse H19 region, we used two sets of primers. Please see below,

The first set : mH19_BSset1F: GAG TAT TTA GGA GGT ATA AGA ATT    mH19_BSset1R:  ATC AAA AAC TAA CAT AAA CCC CT 

The second set: mH19_BSset2F: GTA AGG AGA TTA TGT TTT ATT TTT GG     mH19_BSset2R:  CCT CAT AAA ACC CAT AAC TAT

For more detail of the Tab-sequencing protocol, please see our paper: High-Resolution Analysis of 5-Hydroxymethylcytosine

by TET-Assisted Bisulfite Sequencing, which was publish by our group in 2020.  You would find the paper by this link, https://doi.org/10.1007/978-1-0716-0876-0_25. 

Thanks

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