Code availability

Spatial distribution analysis:

1) Extract salient spatial information from images via segmentation, particle tracking, …

2) Use QuantEv plugin (QuantEv-Densities mode) to extract spatial distribution from different conditions (different genotypes, chemical perturbation, …). These spatial distributions can be visualized via spreadsheet software

3) Use QuantEv plugin (QuantEv-Test mode) to statistically compare the different conditions

Analysis of tracking features with respect to localization:

1) Import particle trajectories extracted from an image sequence in Icy’s TrackManager (possibility to use particle tracker directly in Icy)

2) Use QuantEv TrackProcessor to extract the evolution of a given tracking feature (confinement ratio, displacement distance, total path length or lifetime) with respect to localization from different conditions

3) Use QuantEv plugin (QuantEv-Test mode) to statistically compare the different conditions

Spatial uniformity analysis:

1) Extract salient spatial information from images via segmentation, particle tracking, …

2) Use QuantEv plugin (QuantEv-Uniformity mode) to extract the point giving the most uniform spatial distribution potentially corresponding to a spatial organization hub, emitter, attractor, ...

A tutorial for the Icy plugin is available at http://serpico.rennes.inria.fr/lib/exe/fetch.phpmedia=software:quantev:tutorialforicy.pdf.