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Animals, human tissue, and mouse cells

KA Khaled S. Abd-Elrahman

Last updated date: Jan 28, 2021 Views: 776 Forks: 0

original An abbreviated version of this protocol was published in Science Signaling in Dec, 2020
Aβ oligomers induce pathophysiological mGluR5 signaling in Alzheimer’s disease model mice in a sex-selective manner
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Thanks for your inquiry- I am quoting the perpetration procedure from the manufacturer's product sheet as indicated in the paper

1- Dissolve the lyophilized peptide in HPLC grade (or better) water at 6mg/ml

2- Dilute the peptide to 1mg/ml with PBS (it is important that calcium is omitted)

3- Incubate at 37 C for 24-48 h ( 24-36 h is usually sufficient)

 

 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Abd-Elrahman, K(2021). Animals, human tissue, and mouse cells. Bio-protocol Preprint. bio-protocol.org/prep789.
  2. Abd-Elrahman, K. S., Albaker, A., Souza, J. M. D., Ribeiro, F. M., Schlossmacher, M. G., Tiberi, M., Hamilton, A. and Ferguson, S. S. G.(2020). Aβ oligomers induce pathophysiological mGluR5 signaling in Alzheimer’s disease model mice in a sex-selective manner . Science Signaling 13(662). DOI: 10.1126/scisignal.abd2494

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