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Generation of TILs

SK Sri Krishna

Last updated date: Dec 29, 2020 Views: 995 Forks: 0

original An abbreviated version of this protocol was published in Science in Dec, 2020
Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
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The detailed TIL generation protocol is from reference 50, 51 as described in the supplementary data in the article.

For original articles please see:

(50)  M. E. Dudley, J. R. Wunderlich, T. E. Shelton, J. Even, S. A. Rosenberg, Generation of tumor-infiltrating lymphocyte cultures for use in adoptive             transfer therapy for melanomapatients. J. Immunother. 26, 332–342 (2003).PMID: 12843795

(51)    J. Jin, M. Sabatino, R. Somerville, J. R. Wilson, M. E. Dudley, D. F. Stroncek, S. A. Rosenberg, Simplified method of the growth of human tumor           infiltrating lymphocytes in gas-permeable flasks to numbers needed for patient treatment. J. Immunother. 35, 283–292 (2012).

 


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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Krishna, S(2020). Generation of TILs. Bio-protocol Preprint. bio-protocol.org/prep726.
  2. Krishna, S., Lowery, F. J., Copeland, A. R., Bahadiroglu, E., Mukherjee, R., Jia, L., Anibal, J. T., Sachs, A., Adebola, S. O., Gurusamy, D., Yu, Z., Hill, V., Gartner, J. J., Li, Y. F., Parkhurst, M., Paria, B., Kvistborg, P., Kelly, M. C., Goff, S. L., Altan-Bonnet, G., Robbins, P. F. and Rosenberg, S. A.(2020). Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer. Science 370(6522). DOI: 10.1126/science.abb9847

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Immunology

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I would like to ask about the type of 24-well plate used to culture TILs, any help would be appreciated thanks.
edit 0 Answer 6 Views Mar 6, 2024
This protocol preprint was submitted via the "Request a Protocol" track.