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Acid-base titration

FX Funeng Xu

Last updated date: Dec 4, 2020 Views: 836 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Jul, 2020
Development of a pH-responsive polymersome inducing endoplasmic reticulum stress and autophagy blockade
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CS-poly(β-amino ester) was dispersed in deionized water, and a concentrated HCl aqueous solution was added until the copolymer was completely dissolved. The concentration of the polymer at the moment is 1 mg/ml. The exact pH increases of the solution was monitored with a pH meter at room temperature with a 0.2 M NaOH aqueous solution at an increment of 1-5 μL. Special attention is needed to keep stirring for more than 3 minutes after each titration. Then draw a graph based on the titration results. The pKa of the copolymer was determined as the pH at which 50% of the copolymer turns ionized.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Xu, F(2020). Acid-base titration. Bio-protocol Preprint. bio-protocol.org/prep676.
  2. Xu, F., Li, X., Huang, X., Pan, J., Wang, Y. and Zhou, S.(2020). Development of a pH-responsive polymersome inducing endoplasmic reticulum stress and autophagy blockade . Science Advances 6(31). DOI: 10.1126/sciadv.abb8725

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