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Olfactory receptor neuron (ORN) axotomy assay

MP Maria D Purice

Last updated date: Nov 13, 2020 Views: 1104 Forks: 0

original An abbreviated version of this protocol was published in eLife in Aug, 2017
A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade
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Please see Chapter 24 written by Sean Speese and Mary Logan called "In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster” which has the detailed protocol for ORN axotomy and downstream steps for dissection, staining, and imaging.


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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Purice, M(2020). Olfactory receptor neuron (ORN) axotomy assay. Bio-protocol Preprint. bio-protocol.org/prep625.
  2. Purice, M. D., Ray, A., Münzel, E. J., Pope, B. J., Park, D. J., Speese, S. D. and Logan, M. A.(2017). A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade. eLife. DOI: 10.7554/eLife.23611

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