T-maze, delayed non-match to place version

Detail protocol of the T-maze, delayed non-match to place version

1. This take was conducted using a T-maze constructed to main arm (11 × 56 × 15 cm) and two side arm (11 × 45 × 15 cm).

2. Before all experiments, rats underwent 2 days of habituation, during which they were allowed to freely visit all arms with the food rewards for 10 min.

Note: food reward was used chows with the 1% sucrose water.

3. A sample phase was started after treatments (tDCS or MPH), rats were placed in the starting arm and forced to randomly enter either the right or left arm, receiving a food reward.

Note: Food rewards were performed with only one arm, and this was selected randomly.

4. The test phase was started after a 10-sec delay of a sample phase, both arms were open, and there was one food reward arm (correct) and one empty (error) arm.

Note: Placed the food reward on the opposite side of the sample phase.

Note: Specifies the sample phase (No. 4 of the protocol) and the test phase (No. 4 of the protocol) as the one trial.

5. After each trial, the rat separated by 30 min to the inter-trial interval in the house cage.

6. All animals performed more than seven trials in each session.

Note: We conducted a total of 10 sessions on a tDCS treatment session basis.

7. The index of reward performance was calculated as the average of the correct number of choices.

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