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Codon optimized sequence of human ORC genes

MJ Matt J Jaremko

Last updated date: Oct 12, 2020 Views: 919 Forks: 0

original An abbreviated version of this protocol was published in eLife in Aug, 2020
The dynamic nature of the human origin recognition complex revealed through five cryoEM structures
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I have attached the optimized sequences of the ORC genes 1-5. Keep in mind, ORC1 is truncated on the N-terminus from amino acid 1-470. Also, as mentioned in the paper, we have a strep tactin tag on the N-terminus of ORC3 (not included in sequence).

Related files

txt HsORC1_optimized.txt download
txt HsORC2_optimized.txt download
txt HsORC3_optimized.txt download
txt HsORC4_optimized.txt download
txt HsORC5.1_optimized.txt download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Jaremko, M J(2020). Codon optimized sequence of human ORC genes. Bio-protocol Preprint. bio-protocol.org/prep542.
  2. Jaremko, M. J., On, K. F., Thomas, D. R., Stillman, B. and Joshua-Tor, L.(2020). The dynamic nature of the human origin recognition complex revealed through five cryoEM structures. eLife. DOI: 10.7554/eLife.58622

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