Skin-penetrating IL-RNA complexes

Skin penetrating IL-RNA complexes

IL synthesis

1. The cation, choline bicarbonate and the anion (for eg. geranic acid) was mixed at a 1:2 ratio to prepare IL following salt metathesis reaction.

2. Geranic acid (3.696 mol) was weighed in a round bottom flask equipped with a stirrer and placed into a water/oil bath.

3. Choline bicarbonate 80% solution (1.848 mol) was added dropwise to the flask. The mixture was reacted for 24h at 40°C.

4. The resulting IL solution was dried using a rotary evaporator at 20 mbar at 60 °C for 2 h.

5. The residual water was removed in a vacuum oven at 60 °C for 48 h and characterized.

Preparation of IL-RNA complex

1. ILs were mixed with RNA (100µM) at a volumetric ratio of 1:1 and incubated for 30mins at RT.

2. The RNA-IL solutions (1ml) were dialyzed against 10mM sodium phosphate buffer for 72h using Dialysis Cassettes (10,000 MWCO, Invitrogen).

3. The concentration and stability of RNA were determined.

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