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Flow cytometry antibody panel
Last updated date: Sep 16, 2020 Views: 957 Forks: 0
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Antigen | Clone/Source | Isotype | Fluorochrome | Dilution |
CD11b | M1/70 (BD) | Rat IgG2b, κ | BUV 395 /PercP Cy5.5 | 1/200 |
Ly6C | AL-21 (BD) | Rat IgM, κ | APC-H7 | 1/100 |
Ly6G | 1A8 (BD) | Rat IgG2a, κ | BV421 | 1/500 |
NK1.1 | PK136 (BD) | Mouse IgG2a, κ | PE-Cy7 | 1/100 |
CD45 | 30-F11 (BD) | Rat IgG2b, κ | PercP-Cy5.5 | 1/100 |
CD11c | HL3 (BD) | Armenian Hamster IgG1, λ2 | BV605 | 1/100 |
I-Ab | AF6-120.1 | Mouse IgG2a, κ | APC | 1/100 |
CD62L | MEL-14 (BD) | Rat IgG2a, κ | PE | 1/100 |
Siglec-F | E50-2440 (BD | Rat IgG2a, κ | PE-CF594 | 1/100 |
CD24 | M1/69 (BD) | Rat IgG2b, κ | BV650 | 1/200 |
CD103 | M290 (BD) | Rat IgG2a, κ | BV786 / PE | 1/200 |
F4/80 | BM8 (Biolegend) | Rat IgG2a, κ | A700 | 1/200 |
CD115 | AFS98 (Biolegend) | Rat IgG2a κ
| PE-Cy7 / PE | 1/100 |
CD64 | X54-5/7.1.1 (Biolegend) | Mouse IgG1 κ | PE-Cy7 / PE | 1/500 |
- Boissonnas, A and Loyher, P(2020). Flow cytometry antibody panel. Bio-protocol Preprint. bio-protocol.org/prep504.
- Rodero, M. P., Poupel, L., Loyher, P., Hamon, P., Licata, F., Pessel, C., Hume, D. A., Combadière, C. and Boissonnas, A.(2015). Immune surveillance of the lung by migrating tissue monocytes. eLife. DOI: 10.7554/eLife.07847
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