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Cell culture, nuclei isolation and GRO-seq library preparation

IJ Iris Jonkers
JL John T Lis

Last updated date: Jul 5, 2020 Views: 1198 Forks: 0

original An abbreviated version of this protocol was published in eLife in Apr, 2014
Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons
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Dear Maria,


Our apologies that the source of the Flavopriridol was not clearly stated in the manuscript. We bought it from Sigma Aldrich, in highly purified form. https://www.sigmaaldrich.com/catalog/product/sigma/f3055?lang=en&region=NL


I hope this helps.


Kind regards,

Iris

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Jonkers, I and Lis, J(2020). Cell culture, nuclei isolation and GRO-seq library preparation. Bio-protocol Preprint. bio-protocol.org/prep369.
  2. Jonkers, I., Kwak, H. and Lis, J. T.(2014). Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons. eLife. DOI: 10.7554/eLife.02407

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