MRI experiments

MRI sequence and protocol for "Altered d-glucose in brain parenchyma and cerebrospinal fluid of early Alzheimer’s disease detected by dynamic glucose-enhanced MRI"

Authors: Jianpan Huang, Jiadi Xu*, Kannie WY Chan*

Email: XuJ@kennedykrieger.org;   KannieW.Y.C@cityu.edu.hk

Affiliations:

Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China

Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

This folder contains sequence and protocol for the following paper:

Huang J, van Zijl PCM, Han X, Dong CM, Cheng GWY, Tse K-H, Knutsson L, Chen L, Lai JH, Wu EX, Xu J*, Chan KWY*. Altered D-glucose in brain parenchyma and cerebrospinal fluid of early Alzheimer’s disease detected by dynamic glucose-enhanced MRI. Sci Adv 2020;6(20):eaba3884.

DOI: 10.1126/sciadv.aba3884

If you use this MRI sequence and protocol, please kindly cite our paper mentioned above.

*** Facility requirement ***

MRI scanner: Bruker BioSpec

Software: ParaVision 6

*** How to use ***

The following steps should be done in ParaVision 6, Bruker MRI scanner.

1. For the wave file:

- Copy the ‘bibp.inv’ into the following directory: /opt/PV6.0.1/exp/stan/nmr/lists/wave

2. For the sequence file:

- File-->Import-->Binary Method-->Select vdmpRAREVP_6.0.1.PvUserBinMethod

3. For the protocol file:

- File-->Import-->Protocols-->Select vdmpRAREVP_6.0.1.PvProtocols

*** Notes ***

1. The repetitions should always be even number so that brain parenchyma images and CSF images can be acquired simultaneously.

2. The labeling times are set as 60 ms (pulse number 1 = 4) for parenchyma and 900 ms for CSF (pulse number 1 = 60), respectively, which work well at 3T. Noted that they might be very different at other fields. Usually, the total labeling time is calculated by timing the pulse number and the Bibp pulse width, and is determined by the tissue T2. The flip angle of Bibp is 720 degree (two 360 degree BP pulse).

3. This sequence only works for single slice acquistion.

4. More parameters can be found in the paper mentioned above.

5. For more information about VDMP sequence and other related studies, please visit:

https://pages.jh.edu/~jxu37/resource.html

https://sites.google.com/site/kannienicelab

ALL RIGHTS RESERVED

May 20, 2020