VEGF-A–targeting functional manipulations

VEGF-loaded nanoparticles

Poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) were used for a long term sustained release of VEGF since PLGA NPs have been extensively used and have FDA approval as a vehicle for drug delivery. ¹ NPs containing VEGF or bovine serum albumin (BSA) (0.1 µg/mg of NPs) were prepared using the double emulsion solvent evaporation technique ². The PLGA NP average size was 700 nm, as measured by dynamic light scattering (DLS) technique.¹ The release profile was 40%, 60%, 70% after 6, 13, 21 days respectively, measured by micro bicinchoninic acid (BCA) protein assay kit.

1. X. Jiang et al., Co-delivery of VEGF and bFGF via a PLGA nanoparticle- modified BAM for effective contracture inhibition of regenerated bladder tissue in  rabbits. Sci Rep 6, 20784 (2016).

2. Iqbal M, Zafar N, Fessi H, Elaissari A. Double emulsion solvent evaporation techniques used for drug encapsulation. Int J Pharm. 2015;496:173-90.