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Preprint

Bacterial strains

ZQ Zilu Qu
LY Lei Yin
XZ Xiao-Lian Zhang
ZQ Zilu Qu

Last updated date: Dec 21, 2023 Views: 9 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Oct, 2020
Mycobacterial EST12 activates a RACK1–NLRP3–gasdermin D pyroptosis–IL-1β immune pathway
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Purification of recombinant Mycobacterium tuberculosis protein expressed in prokaryotes: Transform the pet-28a vector of recombinant Mycobacterium tuberculosis gene into E. coli BL-21 strain, 5 ml of  E. coli BL-21 used different concentrations of IPTG and different temperature conditions for protein expression induction, and explored the optimal IPTG induction concentration and temperature.

Copyright: © 2023 The Author(s); This is an open-access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Qu, Z, Yin, L, Zhang, X and Qu, Z(2023). Bacterial strains. Bio-protocol Preprint. bio-protocol.org/prep2538.
  2. Qu, Z., Zhou, J., Zhou, Y., Xie, Y., Jiang, Y., Wu, J., Luo, Z., Liu, G., Yin, L. and Zhang, X.(2020). Mycobacterial EST12 activates a RACK1–NLRP3–gasdermin D pyroptosis–IL-1β immune pathway . Science Advances 6(43). DOI: 10.1126/sciadv.aba4733

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