Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Bacterial strains

ZQ Zilu Qu
LY Lei Yin
XZ Xiao-Lian Zhang
ZQ Zilu Qu

Last updated date: Dec 21, 2023 Views: 22 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Oct, 2020
Mycobacterial EST12 activates a RACK1–NLRP3–gasdermin D pyroptosis–IL-1β immune pathway
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite

Purification of recombinant Mycobacterium tuberculosis protein expressed in prokaryotes: Transform the pet-28a vector of recombinant Mycobacterium tuberculosis gene into E. coli BL-21 strain, 5 ml of  E. coli BL-21 used different concentrations of IPTG and different temperature conditions for protein expression induction, and explored the optimal IPTG induction concentration and temperature.

Copyright: © 2023 The Author(s); This is an open-access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Qu, Z, Yin, L, Zhang, X and Qu, Z(2023). Bacterial strains. Bio-protocol Preprint. bio-protocol.org/prep2538.
  2. Qu, Z., Zhou, J., Zhou, Y., Xie, Y., Jiang, Y., Wu, J., Luo, Z., Liu, G., Yin, L. and Zhang, X.(2020). Mycobacterial EST12 activates a RACK1–NLRP3–gasdermin D pyroptosis–IL-1β immune pathway . Science Advances 6(43). DOI: 10.1126/sciadv.aba4733

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A
This protocol preprint was submitted via the "Request a Protocol" track.