2.8. Observations of seed germination and seedling growth parameters

Dear Sumanta

Hope you will be fine. Detail protocol of your question is already mentioned in section 2.8 along with references. All formulas are also mentioned. 

The sowing day was considered as the first day and the effect of
germination media on seed germination was observed in the morning
every day. 

The total number of germinated seeds was counted every day.
On the other hand, the germination percentage was recorded after 30
days of seedlings. 

The germination percentage was calculated as:
germination % ¼ number of seedlings/total number of seeds x 100. 

Initiation of germination [IoG ¼ seed germination started
day], Speed of germination [SoG ¼ n1/d1 þ n2/d2 þ n3/n3 þ ……
(where, n ¼ number of germinated seeds, d ¼ number of days)], Mean
germination Time [MGT ¼ n1/d1 x n2/d2 x n3/n3 x …… (where, n ¼
number of germinated seeds, d ¼ number of days)], Mean daily germination [MDG ¼ total number of germinated seeds/total number of days],
Peak Value [PV ¼ Highest seed germinated/Number of days], and
Germination values [GV ¼ PV x MDG], completion of germination [CoG
¼ day at which all seeds germinated)] were also calculated (Czabator,
1962; Ellis and Roberts, 1981; ISTA, 1999). 

After 30 days of the experiment, seedling growth parameters such as length of shoot (cm), total no.
of leaves per plant, diameter of leaves (cm), length of leaves (cm), leaf
area (L x B x K) (cm), whole seedling length (cm), root length (cm) were
also recorded.