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CyTOF analysis

CD Chu-Xia Deng

Last updated date: Jul 11, 2023 Views: 725 Forks: 0

original An abbreviated version of this protocol was published in Science Advances
Cullin-5 deficiency orchestrates the tumor microenvironment to promote mammary tumor development through CREB1-CCL2 signaling
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Here are protocols, I followed the procedures by Fluidim. 

We could perform step by step as the file shows, “Procedure for Using Maxpar Pathsetter Templates for CyTOF Acquisition and Analysis” 

We could use the makers to identify immune cells in the file “Maxpar OnDemand Mouse Immune Profiling Panel Kit_TDS-00716”. 

 

Hope they could help you! 

Related files

pdf Maxpar OnDemand Mouse Immune Profiling Panel Kit_TDS-00716.pdf download
pdf Procedure for Using Maxpar Pathsetter Templates for CyTOF Acquisition and Analysis.pdf download
Copyright: © 2023 The Author(s); This is an open-access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Deng, C(2023). CyTOF analysis. Bio-protocol Preprint. bio-protocol.org/prep2363.
  2. Chen, S., Shao, F., Zeng, J., Guo, S., Wang, L., Sun, H., Lei, J. H., Lyu, X., Gao, S., Chen, Q., Miao, K., Xu, X. and Deng, C.(2023). Cullin-5 deficiency orchestrates the tumor microenvironment to promote mammary tumor development through CREB1-CCL2 signaling. Science Advances 9(3). DOI: 10.1126/sciadv.abq1395

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