Purification of DNA Origami Nanostructures

Ultrafiltration using Amicon Ultra 0.5 mL Ultracel filters (50k)
1. Centrifuge 0.5 ml of Fob5 buffer (5 mM TRIS, 1 mM EDTA, 5 mM NaCl, and 5 mM MgCl2) through a filter for 3 min at 25°C, at 10000 g. Discard "flow-through".
2. Add 0.1 - 0.2 ml of folded object sample, fill filter up to 0.5 ml with Fob5 and centrifuge for 3 min at 25°C, at 10000 g. Discard "flow-through".
3. Add 0.45 ml of Fob5 and centrifuge for 3 min at 25°C, at 10000 g. Discard flow through.
4. Repeat step 3, 2 more times.
5. Retrieve sample by placing filter inset upside down into a new tube, centrifuge for 3 min at 25°C, at 10000 g.