Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Study design-Senolytic doses in human livers using proof-of-concept perfusion

SF Stuart J. Forbes
SF Sofia Ferreira-Gonzalez

Last updated date: May 8, 2023 Views: 405 Forks: 0

original An abbreviated version of this protocol was published in Science Translational Medicine in Dec, 2022
Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite
  • Human livers discarded from transplantation were received and prepared for proof-of-concept machine perfusion
  • Upon arrival, the left lateral segments (Couinaud Segments II and III) were split and cannulated using portal vein branches for perfusion. One segment was perfused at 37oC for 2 hours with 1L of sterile Phosphate Buffered Saline (PBS) supplemented with senolytics and the other segment was perfused with PBS and DMSO. 
  • Senolytics were prepared by dissolving 5 mg/kg Dasatinib (LC Laboratories) and 50 mg/kg Quercetin (Sigma-Aldrich) dissolved in 1 ml of DMSO. This mixture was added to the sterile PBS for the senolytic perfusion. 
  • All fluids were recirculated using the perfusion system for the duration of the experiment.
  • Biopsies were obtained prior and after perfusion for the vehicle and senolytic perfused segments
Copyright: © 2023 The Author(s); This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Forbes, S and Ferreira-Gonzalez, S(2023). Study design-Senolytic doses in human livers using proof-of-concept perfusion. Bio-protocol Preprint. bio-protocol.org/prep2279.
  2. Macmillan, M. T., Wang, Y., Mylonas, K. J., Ferenbach, D. A., Kendall, T. J., Lu, W., Acosta, J. C., Kurian, D., O’Neill, S., Oniscu, G. C., Banales, J. M., Krimpenfort, P. J., Forbes, S. J., Boulter, L., Aleksieva, N., Dwyer, B., Gadd, V. L., Campana, L., Younger, N., Rodrigo-Torres, D., Kilpatrick, A. M., Aird, R., Esser, H., Man, T. Y. and Ferreira-Gonzalez, S.(2022). Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage. Science Translational Medicine 14(674). DOI: 10.1126/scitranslmed.abj4375

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A
This protocol preprint was submitted via the "Request a Protocol" track.