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Study design-Senolytic doses in human livers using proof-of-concept perfusion

SF Stuart J. Forbes
SF Sofia Ferreira-Gonzalez

Last updated date: May 8, 2023 Views: 414 Forks: 0

original An abbreviated version of this protocol was published in Science Translational Medicine
Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage
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  • Human livers discarded from transplantation were received and prepared for proof-of-concept machine perfusion
  • Upon arrival, the left lateral segments (Couinaud Segments II and III) were split and cannulated using portal vein branches for perfusion. One segment was perfused at 37oC for 2 hours with 1L of sterile Phosphate Buffered Saline (PBS) supplemented with senolytics and the other segment was perfused with PBS and DMSO. 
  • Senolytics were prepared by dissolving 5 mg/kg Dasatinib (LC Laboratories) and 50 mg/kg Quercetin (Sigma-Aldrich) dissolved in 1 ml of DMSO. This mixture was added to the sterile PBS for the senolytic perfusion. 
  • All fluids were recirculated using the perfusion system for the duration of the experiment.
  • Biopsies were obtained prior and after perfusion for the vehicle and senolytic perfused segments
Copyright: © 2023 The Author(s); This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Forbes, S and Ferreira-Gonzalez, S(2023). Study design-Senolytic doses in human livers using proof-of-concept perfusion. Bio-protocol Preprint. bio-protocol.org/prep2279.
  2. Ferreira-Gonzalez, S., Man, T. Y., Esser, H., Aird, R., Kilpatrick, A. M., Rodrigo-Torres, D., Younger, N., Campana, L., Gadd, V. L., Dwyer, B., Aleksieva, N., Boulter, L., Macmillan, M. T., Wang, Y., Mylonas, K. J., Ferenbach, D. A., Kendall, T. J., Lu, W., Acosta, J. C., Kurian, D., O’Neill, S., Oniscu, G. C., Banales, J. M., Krimpenfort, P. J. and Forbes, S. J.(2022). Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage. Science Translational Medicine 14(674). DOI: 10.1126/scitranslmed.abj4375

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