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Immunofluorescence and imaging
Last updated date: Feb 18, 2020 Views: 1103 Forks: 0
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Laser microirradiation was performed using 405 nm laser under the Zeiss Axiovert 200M Microscope with Marianas Software (Intelligent Imaging Innovations, Inc. Denver, CO, USA).
Settings for laser microirradiation: focus menu > photomanipulation; choose 405 nm laser, adjust power level, duration, raster block size. To aid the inital setting, we used GFP-Ku70, GFP-PARP1 and other DNA repair proteins as positive controls. Laser aliignment should be routinely checked (focus menu > align photomanipulation).
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Peng, A(2020). Immunofluorescence and imaging. Bio-protocol Preprint. bio-protocol.org/prep213.
- Zhu, S., Paydar, M., Wang, F., Li, Y., Wang, L., Barrette, B., Bessho, T., Kwok, B. H. and Peng, A.(2020). Kinesin Kif2C in regulation of DNA double strand break dynamics and repair. eLife. DOI: 10.7554/eLife.53402
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