Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Immunofluorescence and imaging

AP Aimin Peng

Last updated date: Feb 18, 2020 Views: 1082 Forks: 0

original An abbreviated version of this protocol was published in eLife in Jan, 2020
Kinesin Kif2C in regulation of DNA double strand break dynamics and repair
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite

Laser microirradiation was performed using 405 nm laser under the Zeiss Axiovert 200M Microscope with Marianas Software (Intelligent Imaging Innovations, Inc. Denver, CO, USA).

Settings for laser microirradiation: focus menu > photomanipulation; choose 405 nm laser, adjust power level, duration, raster block size. To aid the inital setting, we used GFP-Ku70, GFP-PARP1 and other DNA repair proteins as positive controls. Laser aliignment should be routinely checked (focus menu > align photomanipulation).  

Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Peng, A(2020). Immunofluorescence and imaging. Bio-protocol Preprint. bio-protocol.org/prep213.
  2. Zhu, S., Paydar, M., Wang, F., Li, Y., Wang, L., Barrette, B., Bessho, T., Kwok, B. H. and Peng, A.(2020). Kinesin Kif2C in regulation of DNA double strand break dynamics and repair. eLife. DOI: 10.7554/eLife.53402

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A
This protocol preprint was submitted via the "Request a Protocol" track.