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Monocyte isolation, macrophage differentiation, and OC generation
Last updated date: Dec 28, 2022 Views: 705 Forks: 0
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Thank you for your question. For macrophage differentiation medium, we used MEM alpha supplemented with 10% FBS and 100 U/mL and 100 mg/mL Pen Strep plus 50 ng/mL hMCSF. For OC differentiation medium, we used MEM alpha supplemented with 10% FBS and 100 U/mL and 100 mg/mL Pen Strep plus 20 ng/mL hMCSF and 50 ng/mL hRANKL.
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Wang, Q, Fan, S, Liu, X and Lin, X(2022). Monocyte isolation, macrophage differentiation, and OC generation. Bio-protocol Preprint. bio-protocol.org/prep2096.
- Wang, Q., Wang, H., Yan, H., Tian, H., Wang, Y., Yu, W., Dai, Z., Chen, P., Liu, Z., Tang, R., Jiang, C., Fan, S., Liu, X. and Lin, X.(2022). Suppression of osteoclast multinucleation via a posttranscriptional regulation–based spatiotemporally selective delivery system. Science Advances 8(26). DOI: 10.1126/sciadv.abn3333
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