2.6. Bacterial Zeta Potential

1. Bacteria Z-potential

Fresh and freeze-dried bacteria cultures were prepared and adjusted to a final concentration of 1x10⁸ CFU/mL in double distilled water pH 2.0 (DDW) after rehydration with 1mL DDW for 1 minute at 25°C. The cultures pH was measured and corrected to pH 2.0 by adding HCl 0.1N and 800 ul sample was loaded in DTS1070 cuvettes.  The Smoluchowski model was used to convert electrophoretic mobility data to zeta potential values and it was measured on Zetasizer Nano ZEN 3600 after 2 minutes equilibration time (Malvern Panalytica, UK).