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Preprint

Peritonitis assay

KA Katelyn Arnold
DX Ding Xu
JL Jian Liu

Last updated date: Dec 1, 2022 Views: 881 Forks: 0

original An abbreviated version of this protocol was published in Science Translational Medicine
Design of anti-inflammatory heparan sulfate to protect against acetaminophen-induced acute liver failure
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  1. Inject intraperitoneally mice with 30 mg liver cell lysate in 200 µl PBS
  2. 20 hour later, anesthetize the mice. 
  3. Cut a small opening at the lower abdomen to expose the underneath muscle. Note: Do not compromise the integrity of peritoneal cavity.
  4. Slowly inject 10 ml ice cold PBS with 3mM EDTA into peritoneal cavity using a 26 G needle. Be careful not to penetrate any organ. 
  5. Remove the needle. Message the abdomen of the mice for 1 min to wash peritoneal cavity extensively.
  6. Lay mice on a 10 cm petri dish. Use the tip of sharp scissors, cut a small opening at the center of the abdomen. The PBS will rush out of the peritoneal cavity right away. Grab the head and the tail with each hand, bend the mice to drain out all the PBS into the petri dish. The usual recovery is about 8 ml. 
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Arnold, K, Xu, D and Liu, J(2022). Peritonitis assay. Bio-protocol Preprint. bio-protocol.org/prep2068.
  2. Arnold, K., Xu, Y., Sparkenbaugh, E. M., Li, M., Han, X., Zhang, X., Xia, K., Piegore, M., Zhang, F., Zhang, X., Henderson, M., Pagadala, V., Su, G., Tan, L., Park, P. W., Stravitz, R. T., Key, N. S., Linhardt, R. J., Pawlinski, R., Xu, D. and Liu, J.(2020). Design of anti-inflammatory heparan sulfate to protect against acetaminophen-induced acute liver failure . Science Translational Medicine 12(535). DOI: 10.1126/scitranslmed.aav8075

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