Mice

Dear Dr. Guo,

I hope this email finds you well!

Thank you for your interest in our work. I would like to address the two questions you have as follows:

1. The OGT knockout models are VMH neuron-specific OGT knockout, rather than whole-body knockout. We used the Cre-loxp system to generate mouse models with VMH neuron-specific OGT knockout. We used two approaches: 1) Cross Sf1-Cre mice with OGT floxed mice to generate the mice with prenatal OGT deletion in VMH neurons (SF1 as the marker); 2) Apply stereotaxic virus injection to inject AAV-Cre virus into the VMH of OGT floxed mice to generate the mice with postnatal OGT deletion in VMH neurons.
    
2. The white adipose tissues and brown adipose tissues were distinguished and collected separately during the dissection of mice. Since they are located in different positions across the body of mice and have different morphological appearances, they are relatively easy to be distinguished. More information regarding the dissection of adipose tissues can be found in previous publications: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7017470/.

I hope our answers are helpful. Please feel free to let us know should you have further questions. Thank you!

Best wishes,

Qi