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Preprint

spGFP11::2HA::Ort nucleotide sequence

TL Tzu-Yang Lin
CL Chi-Hon Lee

Last updated date: Sep 28, 2022 Views: 509 Forks: 0

original An abbreviated version of this protocol was published in eLife in Mar, 2020
Antagonistic regulation by insulin-like peptide and activin ensures the elaboration of appropriate dendritic field sizes of amacrine neurons
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LOCUS       spGFP11::2HA::Ort                 1584 bp ds-DNA     linear       29-SEP-2022

FEATURES             Location/Qualifiers
    misc_feature    join(1..85,212..1584)
                    /locus_tag="ORT CDS"
  
    misc_feature    142..198
                    /locus_tag="2HA"
                    
    misc_feature    88..135
                    /locus_tag="sp11"   
                    
ORIGIN
       1 ATGGTGTTCC AAATAATAAT CCTGGTGATC TGCACCATCT GCATGAAGCA CTACGCCAAA
      61 GGGGAGTTTC AACAAAGTCT GGCCAtaCGC GACCACATGG TGCTGCACGA GTACGTGAAC
     121 GCCGCCGGCA TCACCGGCTC CTACCCCTAC GACGTGCCCG ACTACGCCGG CTATCCCTAT
     181 GACGTCCCGG ACTATGCAGG CTCCtTGGCC ATAACCGACA TCCTGCCCGA GGACATCAAG
     241 CGTTACGACA AGATGAGACC GCCGAAGAAA GAGGGTCAGC CGACGATAGT CTACTTCCAT
     301 GTGACGGTGA TGGGTCTGGA CTCCATTGAT GAGAACTCGA TGACTTATGT GGCGGATGTG
     361 TTCTTTGCAC AGACGTGGAA GGATCATCGC CTGCGATTGC CGGAGAATAT GACACAGGAA
     421 TACCGCCTGC TCGAGGTGGA CTGGCTAAAA AATATGTGGC GGCCGGATTC GTTTTTCAAA
     481 AACGCCAAAT CGGTGACCTT TCAGACCATG ACAATACCCA ATCACTATAT GTGGCTGTAC
     541 AAGGATAAGA CCATTCTGTA CATGGTCAAA CTAACACTGA AGCTGTCCTG CATCATGAAC
     601 TTCGCCATTT ATCCTCATGA TACACAGGAG TGCAAGCTGC AAATGGAAAG CCTGTCCCAC
     661 ACCACGGATG ACTTGATATT CCAGTGGGAT CCAACAACGC CCCTTGTGGT TGATGAAAAC
     721 ATCGAACTGC CGCAGGTGGC CCTCATCCGA AATGAAACGG CGGACTGTAC CCAAGTTTAT
     781 TCCACTGGCA ACTTCACATG CCTGGAGGTG GTGTTCACCC TTAAGCGTCG TTTGGTTTAC
     841 TACGTTTTCA ACACCTACAT TCCCACCTGC ATGATAGTGA TCATGTCATG GGTATCCTTC
     901 TGGATCAAAC CGGAGGCGGC ACCAGCCCGT GTGACTCTGG GTGTCACCTC CTTGCTAACG
     961 CTTTCCACGC AACACGCCAA ATCGCAGTCG TCTTTGCCAC CTGTTTCCTA TCTCAAGGCA
    1021 GTGGACGCCT TTATGTCCGT TTGCACGGTG TTCGTGTTTA TGGCCCTCAT GGAGTATTGT
    1081 CTAATAAACA TCGTCCTGAG CGACACGCCC ATTCCCAAGC CGATGGCCTA TCCACCCAAA
    1141 CCTGTGGCGG GAGATGGGCC CAAGAAAGAG GGCGAGGGTG CTCCTCCTGG GGGCAGCAAC
    1201 TCGACGGCCA GCAAGCAACA AGCCACCATG TTGCCACTGG CCGATGAAAA GATCGAGAAA
    1261 ATTGAGAAGA TCTTTGACGA GATGACCAAG AATAGAAGGA TTGTAACCAC CACACGCCGC
    1321 GTGGTGCGTC CGCCATTGGA CGCCGATGGT CCGTGGATTC CGCGACAGGA GTCGCGGATA
    1381 ATACTGACCC CGACTATCGC TCCGCCGCCA CCGCCCCCTC AGCCAGCGGC ACCGGAACCG
    1441 GAACTGCCCA AGCCGAAACT CACACCCGCC CAGGAGCGGC TCAAGCGGGC TATATATATA
    1501 GATCGGTCCT CGCGCGTCCT TTTCCCCGCC CTCTTCGCCA GTCTGAATGG CATCTACTGG
    1561 TGTGTGTTCT ACGAGTATCT ATAA   
//
 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Lin, T and Lee, C(2022). spGFP11::2HA::Ort nucleotide sequence. Bio-protocol Preprint. bio-protocol.org/prep1970.
  2. Luo, J., Ting, C., Li, Y., McQueen, P., Lin, T., Hsu, C. and Lee, C.(2020). Antagonistic regulation by insulin-like peptide and activin ensures the elaboration of appropriate dendritic field sizes of amacrine neurons. eLife. DOI: 10.7554/eLife.50568

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