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Synthesis and characterization of ROS-scavenging hydrogel

YG Ya Guan
HN Hong Niu
JG Jianjun Guan

Last updated date: Sep 22, 2022 Views: 551 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Aug, 2021
Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
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  1. Dissolve NIPAAm, HEMA and 4-(acryloxymethyl)-phenylboronic acid pinacol ester in 1, 4-dioxane at a molar ratio of 75/15/10. 

2. Purge the solution with N2 for 30 min to remove air.

3. Add benzoyl peroxide to the solution. The molar ratio of benzoyl peroxide and all monomers is 1:500.

4. Purge the solution with N2 for another 30 min.

5. Immerse the flask with the above solution in a 70oC oil bath overnight with continuous stirring.

6. Precipitate the solution into cold hexane. 

7. Dissolve the polymer in tetrahydrofuran.

8. Precipitate the polymer solution into cold diethyl ether.

9. Repeat steps 8 and 9 twice.

10. Air dry the polymer followed by vacuum drying at 40oC for 1 day.

11. The dry polymer is dissolved in CDCl3 to determine the chemical structure using an 1H-NMR spectroscopy.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Guan, Y, Niu, H and Guan, J(2022). Synthesis and characterization of ROS-scavenging hydrogel. Bio-protocol Preprint. bio-protocol.org/prep1960.
  2. Guan, Y., Niu, H., Liu, Z., Dang, Y., Shen, J., Zayed, M., Ma, L. and Guan, J.(2021). Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation. Science Advances 7(35). DOI: 10.1126/sciadv.abj0153

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