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Yeast strains and growth conditions

PK Pil Jung Kang
HP Hay-Oak Park

Last updated date: Jul 27, 2022 Views: 535 Forks: 0

original An abbreviated version of this protocol was published in Molecular Biology of the Cell in Mar, 2022
Up-regulation of the Cdc42 GTPase limits the replicative life span of budding yeast
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GFP-Cdc42 was expressed from the PCDC42-GFP-linker-CDC42 (linker: APPRRLVHP) targeted to the URA3 locus (using YIplac211-PCDC42-GFP-linker-CDC42). This strain and another strain that carries the extra copies of GFP-CDC42 were originally described in Kuo et al. (2014) Cure Biol 24:753–759. The endogenous CDC42 was deleted by crossing with DLY13891 (MATa cdc42::TRP1 URA3:GFP-CDC42 (8x) in the same genetic background) (Wu et al., 2015, eLife 4, 11611). As cited in the text (see the details in Wu et al., 2015), these fusions are not fully functional at a temperature higher than 34oC.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Kang, P and Park, H(2022). Yeast strains and growth conditions. Bio-protocol Preprint. bio-protocol.org/prep1822.
  2. Kang, P. J., Mullner, R., Li, H., Hansford, D., Shen, H. and Park, H.(2022). Up-regulation of the Cdc42 GTPase limits the replicative life span of budding yeast. Molecular Biology of the Cell 33(4). DOI: 10.1091/mbc.E21-04-0208

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