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Preprint

HLA Typing by High-Resolution Next-Generation Sequencing

RW Roos A. W. Wennink
JK Jonas J. W. Kuiper

Last updated date: Jul 13, 2022 Views: 568 Forks: 0

original An abbreviated version of this protocol was published in Investigative Ophthalmology & Visual Science
Next-Generation HLA Sequence Analysis Uncovers Shared Risk Alleles Between Clinically Distinct Forms of Childhood Uveitis
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The protocol for the library preparation for next-gen seq and HLA amplification for HLA-A, -B, -C, DRB1, DQB1, and DPB1 are provided in the pdf files. 

Related files

pdf ifu-ngsgo-library-full-kit-2812006-edition-4-2022-06-ce-ivdd-ivdr.pdf download
pdf ifu-ngsgo-mx6-1-7910004-edition-3-2022-06-ce-ivdr.pdf download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Wennink, R and Kuiper, J(2022). HLA Typing by High-Resolution Next-Generation Sequencing. Bio-protocol Preprint. bio-protocol.org/prep1793.
  2. Wennink, R. A. W., Boer, J. H. D., Hiddingh, S., Haasnoot, A. J. W., Ayuso, V. K., Hoop, T. D., Setten, J. V., Spierings, E. and Kuiper, J. J. W.(2021). Next-Generation HLA Sequence Analysis Uncovers Shared Risk Alleles Between Clinically Distinct Forms of Childhood Uveitis. Investigative Ophthalmology & Visual Science 62(9). DOI: 10.1167/iovs.62.9.19

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