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Preprint

IVDi culture

TY Takashi Yoshino
KH Katsuhiko Hayashi

Last updated date: Jun 28, 2022 Views: 600 Forks: 0

original An abbreviated version of this protocol was published in Science in Jul, 2021
Generation of ovarian follicles from mouse pluripotent stem cells
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Thank you very much for your question.

We used Transwell-COL membrane insert (Costar, cat. no. 3492).

However, it is currently out of stocks.

Therefore, we try to find other Transwell-COL membrane insert.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Yoshino, T and Hayashi, K(2022). IVDi culture. Bio-protocol Preprint. bio-protocol.org/prep1755.
  2. Yoshino, T., Suzuki, T., Nagamatsu, G., Yabukami, H., Ikegaya, M., Kishima, M., Kita, H., Imamura, T., Nakashima, K., Nishinakamura, R., Tachibana, M., Inoue, M., Shima, Y., Morohashi, K. and Hayashi, K.(2021). Generation of ovarian follicles from mouse pluripotent stem cells. Science 373(6552). DOI: 10.1126/science.abe0237

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