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Preprint

Staining and microscopy

ZM Zhipeng Meng
FL Fu-Long Li
KG Kun-Liang Guan

Last updated date: Jun 27, 2022 Views: 573 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in May, 2022
The Hippo pathway mediates Semaphorin signaling
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Please see the list below. Thanks for your interest! - Zhipeng

 

PlexinA2          Cell Signaling Technology       5658

PlexinA3          Cell Signaling Technology       5512

ARHGAP5        Cell Signaling Technology       2562

ARHGAP35      Cell Signaling Technology       12164

YAP (phos-tag) Cell Signaling Technology       14074

LATS1             Cell Signaling Technology       3477

LATS2              Cell Signaling Technology       5888

pLATS1            Cell Signaling Technology       8654

MYC-tag          Cell Signaling Technology       2276

HA-HRP           Cell Signaling Technology       2999

 

YAP/TAZ          Santa Cruz Technology           sc-101199

GAPDH            Santa Cruz Technology           sc-25778

 

Flag-HRP         Sigma                                      A8592 

Flag (for IP)     Sigma                                     F1804 (for IP)

 

NRP2               R&D System                             AF2215

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Meng, Z, Li, F and Guan, K(2022). Staining and microscopy. Bio-protocol Preprint. bio-protocol.org/prep1745.
  2. Meng, Z., Li, F., Fang, C., Yeoman, B., Qiu, Y., Wang, Y., Cai, X., Lin, K. C., Yang, D., Luo, M., Fu, V., Ma, X., Diao, Y., Giancotti, F. G., Ren, B., Engler, A. J. and Guan, K.(2022). The Hippo pathway mediates Semaphorin signaling. Science Advances 8(21). DOI: 10.1126/sciadv.abl9806

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