We followed the protocol that had been previously published by Hughes et al. 2012. You can find more details in their paper: doi:10.1101/gr.128876.111
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How to cite:
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
Martin Anduaga, A and Kadener, S(2022). Total RNA-seq. Bio-protocol Preprint. bio-protocol.org/prep1700.
Martin Anduaga, A., Evantal, N., Patop, I. L., Bartok, O., Weiss, R. and Kadener, S.(2019). Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila. eLife. DOI: 10.7554/eLife.44642
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