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Preprint

Comparative metabolic profiling

JY Jin-Ming Yang
JS Jianxun Song

Last updated date: Apr 8, 2022 Views: 575 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Feb, 2022
Elongation factor-2 kinase is a critical determinant of the fate and antitumor immunity of CD8+ T cells
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Seahorse Xfe96 fluxpaks (plate and cartridge) - 102416-100

Seahorse XF glycolytic rate assay kit - 103344-100

The RPMI medium used for the study was without phenol red and catalog number was: 103336-100.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Yang, J and Song, J(2022). Comparative metabolic profiling. Bio-protocol Preprint. bio-protocol.org/prep1611.
  2. Das, J. K., Ren, Y., Kumar, A., Peng, H., Wang, L., Xiong, X., Alaniz, R. C., de Figueiredo, P., Ren, X., Liu, X., Ryazonov, A. G., Yang, J. and Song, J.(2022). Elongation factor-2 kinase is a critical determinant of the fate and antitumor immunity of CD8+ T cells. Science Advances 8(5). DOI: 10.1126/sciadv.abl9783

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