Knockdown of FBN1 in vivo

Knockdown of FBN1 in vivo

Male C57/BL6 mice weighing 21–24 g were divided into three groups (n=6 in each group): (i) sham-operated mice, (ii) UIRI mice injected with control shRNA, and (iii) UIRI mice injected with FBN1-shRNA. Four days after IRI, mice were injected with either pLVX-shFBN1 or control (pLVX-control) plasmids via tail vein injection using hydrodynamics-based gene transfer approach. Plasmids were diluted with sterile PBS. The amount of plasmid for mice was 1 mg per kg body weight. The fluid volume via tail-vein injection of each mouse is about 10% of the body weight, and FBN1 or control shRNA should be rapidly administered (within 10 seconds) into mice via tail-vein injection. Mice were euthanized at 11 days after IRI, and serum and kidney tissues were analyzed.

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