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2.2.2. Maceration Extraction (ME)
Last updated date: Mar 6, 2022 Views: 577 Forks: 0
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- 0.3 ± 0.001 g of dried and milled flower heads were macerated in 10 mL ethanol of selected concentration ethanol (50 or 70 % v/v).
- After macerating samples for 12 hours, they had to be centrifuged for 10 min at 3382 g in cenrifuge, followed by the decantation of the supernatant.
- The extracts were hydrolyzed using alkaline hydrolysis - alkali hydrolysis was carried out using 25% NaOH. The samples pH was changed to 10.5 and then the extracts were sonicated at 45 ± 2 °C for 10 min.
- After hydrolysis, the sample was neutralized to pH 5.7 using 25% acetic acid.
- After hydrolysis samples were filtered through PVDF syringe filters (pore size 0.22 μm) for further HPLC analysis.
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Kazlauskaite, J and Bernatoniene, J(2022). 2.2.2. Maceration Extraction (ME). Bio-protocol Preprint. bio-protocol.org/prep1566.
- Kazlauskaite, J. A., Ivanauskas, L. and Bernatoniene, J.(2021). Novel Extraction Method Using Excipients to Enhance Yield of Genistein and Daidzein in Trifolium pratensis L.. Pharmaceutics 13(6). DOI: 10.3390/pharmaceutics13060777
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