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Preprint

Mouse breeding and husbandry

TB Trygve E Bakken
Cv Cindy TJ van Velthoven

Last updated date: Dec 14, 2021 Views: 729 Forks: 0

original An abbreviated version of this protocol was published in eLife in Sep, 2021
Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans
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Please reference the following protocols:

  • Slice Preparation with Tissue Dissociation - Mouse Protocol V.2 dx.doi.org/10.17504/protocols.io.bq6wmzfe
  • Isolation of Nuclei from Adult Mouse Brain Tissue Protocol V.2 dx.doi.org/10.17504/protocols.io.bq7emzje
  • FACS Single Cell Sorting V.4 dx.doi.org/10.17504/protocols.io.be4cjgsw
  • SMARTerV4 (0.5x) Amplification for single-cell or single-nuclei RNASeq Protocol dx.doi.org/10.17504/protocols.io.7d5hi86
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Bakken, T E and van Velthoven, C T(2021). Mouse breeding and husbandry. Bio-protocol Preprint. bio-protocol.org/prep1476.
  2. Bakken, T. E., van Velthoven, C. T., Menon, V., Hodge, R. D., Yao, Z., Nguyen, T. N., Graybuck, L. T., Horwitz, G. D., Bertagnolli, D., Goldy, J., Yanny, A. M., Garren, E., Parry, S., Casper, T., Shehata, S. I., Barkan, E. R., Szafer, A., Levi, B. P., Dee, N., Smith, K. A., Sunkin, S. M., Bernard, A., Phillips, J., Hawrylycz, M. J., Koch, C., Murphy, G. J., Lein, E., Zeng, H. and Tasic, B.(2021). Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans. eLife. DOI: 10.7554/eLife.64875

Category

Zoology

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