Attached is a detailed protocol for the histone proteomics prep. This protocol, as well as other templates for histone analysis, are also included in the MassIVE dataset published with the paper (MSV000087800) in the "Methods and Protocols" folder. Good luck!
Related files
Histone_Prep_for_Mass_Spec.docx 
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How to cite:Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Thomas, S and Denu, J(2021). Histone proteomics. Bio-protocol Preprint. bio-protocol.org/prep1447.
- Thomas, S. P. and Denu, J. M.(2021). Short-chain fatty acids activate acetyltransferase p300. eLife. DOI: 10.7554/eLife.72171
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