Flow cytometry, FACS, and CyTOF

For detection of KIRs on uterine NK cells several antibody combinations have been used, depending on the purpose of the staining. In case of bulk panKIR staining for flow cytometry, antibodies detecting KIR2DL1/S1 (clone EB6, Beckman Coulter) and KIR2DL2/L3/S2 (clone GL183, Beckman Coulter) were combined. To identify single KIRs and their respective combinations of expression, antibodies detecting KIR2DL2/L3/S2 (clone CH-L, BD Biosciences), KIR2DL1 (Miltenyi, clone used in publication now discontinued, currently clone REA284 available), KIR3DL1 (DX9, BD biosciences), KIR2DS4 (JJC11.6, MIiltenyi), and KIR2DL3 (Miltenyi, clone used in publication now discontinued, currently clone REA147 available) were used. See also Supplementary table S5 for list of reagents/antibodies used. Staining of KIRs for CyTOF was performed with the antibodies as indicated in table S5. Here, antibodies detecting KIR2DL4 (clone mAb 33, BioLegend), KIR2DL3 (clone 180701, R&D Systems), KIR2DL1 (clone 143211, R&D Systems), KIR3DL1 (clone DX9, LSBio), KIR3DL1/S1 (clone Z27.3.7, Beckman Coulter), and KIR2DL2/L3/S2 (clone GL183, Biorad) were used. 

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